Biomedical Engineering
Below are some images and videos about BME stuff, mainly from my work in the cell and tissue engineering lab.
In a short experiment, migration of HeLa cells was investigated by means of a scratch assay. Using pipet tips, scratches were made on a monolayer of confluent HeLa cells. Migration of cells into the scratch was assessed after 6 and 14 hours. Different parts of the cytoskeleton were stained: F-actin was stained with TRITC-labeled phalloidin, vimentin (an intermediate filament protein) was stained using a specific antibody bound to Alexa 488. DAPI was used to stain nuclei. The images below were taken using a confocal microscope (Zeiss LSM510 META NLO).
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t=6hM=20x |
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t=14hM=10x |
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Next, some images of the same experiment taken with a normal fluorescence microscope (Zeiss Axiovert 200M).
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t=14hM=63x |
A video of human mesenchymal stem cells embedded in a collagen hydrogel. Live cells are stained green (calcein-AM), dead cell's nuclei are stained red (propidium iodide). Small green dots are fluorescent microbeads (ø1 um). Image slices were taken on a confocal microscope and converted into a video. Depth into the hydrogel (from bottom upwards) is displayed in the top left corner. Scale bar: 100 um.